Sudan Black B (C 29 24 N 6 ) is nonfluorescent lysochrome, relatively thermostable (fat soluble) dye) diazo dye is used for the staining of neutral triglycerides and lipids in the frozen section and some lipoproteins in the paraffin portion. It has a dark brown powder appearance to black with maximum absorption at 596-605Ã,m and a melting point of 120-124Ã, Â ° C. The color is blackish.
Sudan Black B is one of the dyes used for Sudanese staining. Similar dyes include Red O Oil, Sudan III, and Sudan IV.
Sudan Black B can be used to color some materials other than other Sudanese dyes, because it is not so specific to lipids.
Use of Sudan Black B in fingerprint enhancement. This is useful for detecting fats contaminated with oils and fats.
In distinguishing the black Sudanese hematological disorder will stain the myeloblast but not the lymphoblast.
Sudan Black B was introduced by Lison and Dagnelie in 1935.
Video Sudan Black B
Production and composition
Sudan Black is formed by the diazotization clutch 4-phenylazo-1-naphthylamine with 2,3-dihydro-2,2-dimethyl-1 H -perimidine. The main product therefore is expected to be 2,3-dihydro-2,2dimethyl-6 - [(4-phenylazo-1-naphthalenyl) -azo] -1 H -perimidine. However, the dyes produced from the above reaction product actually contain many, up to 42 colorless and fractionally colorless byproducts. Two main products of blue are confirmed by various chromatography (TLC and column etc.) separation and spectroscopic identification (IR, NMR, Mass) are named SBB-I & amp; SBB-II (Rf values ​​0.49 and 0.19 (chloroform/benzene 1: 1, SiO2) in Thin Layer Chromatography). The product described above is indeed the SSB-II comprising up to 60% of the mixture, and SBB-I is 2,3-dihydro-2,2-dimethyl-4- [(4-phenylazo-1- naphthalenyl) -azo ] -1 H -perimidine.
Maps Sudan Black B
References
External links
- File entry stains
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